Humaan dihydrofolaat Reductase/DHFR antilichaam

Dihydrofolate reductase (DHFR) catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of DNA synthesis and cell death. Gene expression of methotrexate (MTX) -resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.

Dihydrofolate reductase, EC 1.5.1.3

Human

Human

Recombinant human DHFR (1-187aa) purified from E. coli

AT5B2

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Dihydrofolate Reductase, DHFR, Dihydrofolate reductase, EC 1.5.1.3, ATGA0376-10 µg, ATGA0376-20 µg, ATGA0376-50 µg, ATGA0376-100 µg, ATGA0376-250 µg, ATGA0376-500 µg, ATGA0376-1 mg, ATGA0376-10, ATGA0376-20, ATGA0376-50, ATGA0376-100, ATGA0376-250, ATGA0376-500, ATGA0376-1

Chen MJ., et al. (1984) J Biol Chem. 259 (6) : 3933-3943. ; ; Cody V., et al. (2009) Biochemistry. 48 (8) : 1702-1711.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Other

NP_000782

P00374

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : Jurkat cell lysate ; ; Lane 3 : 293T cell lysate

ICC/IF analysis of DHFR in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DHFR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .