Humaan E2-EPF/UBE2S antilichaam

UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins.

Ubiquitin-conjugating enzyme E2S, E2-EPF, E2EPF, EPF5, Ubiquitin-conjugating enzyme S, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S, Ubiquitin conjugating enzyme E2 24 kD, Ubiquitin conjugating enzyme E2S

Human

Human

Recombinant human UBE2S (1-222aa) purified from E. coli

AT2C12

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

UBE2S, ATGA0165-10 µg, ATGA0165-20 µg, ATGA0165-50 µg, ATGA0165-100 µg, ATGA0165-250 µg, ATGA0165-500 µg, ATGA0165-1 mg, ATGA0165-10, ATGA0165-20, ATGA0165-50, ATGA0165-100, ATGA0165-250, ATGA0165-500, ATGA0165-1

Ye Y, et al. (2009) Nat Rev Mol Cell Biol, 10 (11) :755-64.; ; Windheim M, et al. (2008) Biochem J, 409 (3) :723-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Ubiquitination

NP_055316.2

Q16763

The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2S antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: UBE2S Transfected 293T cell lysate;

Flow cytometry analysis of UBE2S in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human UBE2S antibody (1:500) with Alexa 488 (Green) .