Humaan EGFR antilichaam

Epidermal growth factor receptor (EGFR) is a 170 kDa membrane protein first identified as a binding partner of EGF. Also, EGFR phosphorylated itself (autophosphorylation) in response to EGF treatment. The overexpression of EGFR in various epithelial tumors was widely reported and it has been substantiated that deregulation of EGFR itself and its signaling pathways an important role in human cancers.

Epidermal growth factor receptor, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, ERRP, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian)

Human

Human

Recombinant human EGFR (424-605aa) purified from E. coli

AT2H8

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

EGFR, ATGA0212-10 µg, ATGA0212-20 µg, ATGA0212-50 µg, ATGA0212-100 µg, ATGA0212-250 µg, ATGA0212-500 µg, ATGA0212-1 mg, ATGA0212-10, ATGA0212-20, ATGA0212-50, ATGA0212-100, ATGA0212-250, ATGA0212-500, ATGA0212-1

Cohen S, (1964) Nat Cancer Inst Monogr, 13: 13-27. ; ; Toyoda H, et al. (1995) J Biol Chem, 270: 7495-500. ; ; Carpenter G, et al. (1978) Nature,276: 409-10.

Hoshi H, et al. Construction of a novel cell-based assay for the evaluation of anti-EGFR drug efficacy against EGFR mutation. (Oncol Rep. 2017) {https://pubmed.ncbi.nlm.nih.gov/27840973/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_005219

P00533

The cell lysates of HaCaT were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR (1:250) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) or phalloidin-TRITC for nucleus or actin staining, respectably. monoclonal anti-human EGFR antibody (1:250) was stained with alexa 488 (green) .