Humaan EMAP-II/AIMP1-antilichaam

AIMP1, also known as p43, hasn't until recently been recognized for its prominent immunological functions. Together with other nonenzymatic mARS structural components AIMp2/38 and AIMp3/p18, it participates in the machinery responsible for cell-cycle control and tumor suppression.

Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, EMAP2, EMAPII, p43, SCYE1

Human

Human

Recombinant human AIMP1 (1-336aa) purified from E. coli

AT6D10

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

EMAP-2, AIMP1, ATGA0396-10 µg, ATGA0396-20 µg, ATGA0396-50 µg, ATGA0396-100 µg, ATGA0396-250 µg, ATGA0396-500 µg, ATGA0396-1 mg, ATGA0396-10, ATGA0396-20, ATGA0396-50, ATGA0396-100, ATGA0396-250, ATGA0396-500, ATGA0396-1

Liang D., et al. (2015) Int Rev Immunol. 34 (5) : 367-381.; ; Hong SH., et al. (2015) Biomaterials. 44: 45-54.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Apoptosis

NP_001135888

Q12904

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIMP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : HepG2 cell lysate; ; Lane 3. : Raji cell lysate ; ; Lane 4. : Jurkat cell lysate ; ; Lane 5. : A549 cell lysate ; ; Lane 6. : HaCaT cell lysate

Flow cytometry analysis of AIMP1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of AIMP1 in Hep3B cells. The cell was stained with ATGA0396 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .