Humaan Enolase 2/ENO2-antilichaam

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma.

Enolase 2 (gamma, neuronal), ENO2, NSE, Neuron-Specific Enolase, 2 phospho D glycerate hydrolyase, Eno 2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma enolase, Neural enolase, Neuron specific enolase, Neuron specific gamma enolase, Neurone specific enolase.

Human

Human

Recombinant human NSE (1-434aa) purified from E. coli

AT17D10

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Enolase2, Neuron-specific Enolase, Enolase 2 (gamma, neuronal), ENO2, NSE, Neuron-Specific Enolase, 2 phospho D glycerate hydrolyase, Eno 2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma enolase, Neural enolase, Neuron specific enolase, Neuron specific gamma enolase, Neurone specific enolase., ATGA0273-10 µg, ATGA0273-20 µg, ATGA0273-50 µg, ATGA0273-100 µg, ATGA0273-250 µg, ATGA0273-500 µg, ATGA0273-1 mg, ATGA0273-10, ATGA0273-20, ATGA0273-50, ATGA0273-100, ATGA0273-250, ATGA0273-500, ATGA0273-1

Oliva D, Cali L, Feo S, Giallongo A (1991) . Genomics 10 (1) : 157-65.; ; Craig SP, Day IN, Thompson RJ, Craig IW (1991) . Cytogenet. Cell Genet. 54 (1-2) : 71-3.; ; McAleese SM, Dunbar B, Fothergill JE, et al. (1989) . Eur. J. Biochem. 178 (2) : 413-7.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000.

Neuroscience

NP_001966

P09104

The extracts of mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:500 ~ 1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: NSE Transfected 293T cell lysate {ATGA0273-WB2.jpg} The Cell lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: U-87 MG cell lysate ; ; Lane 2.: Jurkat cell lysate {ATGA0273-WB3.jpg} The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSE antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant protein ENO1 ; ; Lane 2.: Recombinant protein NSE (ENO2) ; ; Lane 3.: Recombinant protein ENO3 {ATGA0273-WB4.jpg}

ICC/IF analysis of NSE in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NSE antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .