Humaan Epoxide Hydrolase 1/EÜX1 antilichaam

Epoxide hydrolase 1 (EPHX1) is a family of biotransformation enzyme which is involved in the hydrolysis of various epoxides and epoxide intermediates. Epoxide hydrolase plays an important role in both the activation and detoxification of exogenous chemicals such as polycyclic aromatic hydrocarbons.

Epoxide hydrolase 1, Epoxide hydrolase 1 microsomal (xenobiotic), MEH, EPHX, EPOX

Human

Human

Recombinant human EPHX1 (21-455aa) purified from E. coli

AT2E5

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Microsomal (xenobiotic), MEH, Epoxide hydrolase 1 microsomal (xenobiotic), Epoxide hydrolase 1, EPOX, EPHX1, EPHX, ATGA0143-10 µg, ATGA0143-20 µg, ATGA0143-50 µg, ATGA0143-100 µg, ATGA0143-250 µg, ATGA0143-500 µg, ATGA0143-1 mg, ATGA0143-010, ATGA0143-020, ATGA0143-050, ATGA0143-100, ATGA0143-250, ATGA0143-500, ATGA0143-01M

Bertvan Loo, et al., (2006) Appl Environ Microbiol 72 (4) :2905-2917.; ; Janet K.C, et al., (2001) The Histochemical Journal 33:329-336.; ; Hassett C, et al., (1994) Genomics 23 (2) :433-442.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_000111

P07099

The tissue lysate (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHX1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Liver Tissue lysateThe cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHX1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: EPHX1 Transfected 293T cell lysate; ; {ATGA0143-WB2.jpg}

ICC/IF analysis of EPHX1 in Hep3B cells. The cell was stained with ATGA0143 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .