Humaan erytropoëtine/EPO-antistoffen

EPO, also known as erythropoietin, is a glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types.

EPO, EP, MVCD2

Human

Human

Recombinant human Erythropoietin (28-193aa) purified from insect cell

AT1C12

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Erythropoietin, ATGA0512-10 µg, ATGA0512-20 µg, ATGA0512-50 µg, ATGA0512-100 µg, ATGA0512-250 µg, ATGA0512-500 µg, ATGA0512-1 mg, ATGA0512-10, ATGA0512-20, ATGA0512-50, ATGA0512-100, ATGA0512-250, ATGA0512-500, ATGA0512-1

Koury, M.J., et al. (2005) Exp. Hematol. 33:1263.; ; Jelkmann W., et al. (2007) Eur J Haematol. 78:183-205.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Angiogenesis

NP_000790

P01588

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPO antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: EPO transfected 293T cell lysate