Humaan FABP1/L-FABP antilichaam

FABP1 (Fatty acid binding protein1) encodes the fatty acid binding protein found in liver. FABP1 is composed of ten antiparallelbeta strands that form a barrel with a bigger binding pocket than the other FABPs allowing it to accommodate two fatty acid. This protein binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm; it may be involved in intracellular lipid transport and metabolism.

Fatty acid binding protein 1, Fatty acid-binding protein, liver, FABPL, L-FABP, FABP1, Fatty acid binding protein 1, fatty acid binding protein 1 liver

Human

Human

Recombinant human FABP1 (1-127aa) purified from E. coli

2G4

Unconjugated

ELISA, WB, ICC/IF, IHC, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

FABP1, L-FABP, AFA0908-10 µg, AFA0908-20 µg, AFA0908-50 µg, AFA0908-100 µg, AFA0908-250 µg, AFA0908-500 µg, AFA0908-1 mg, AFA0908-10, AFA0908-20, AFA0908-50, AFA0908-100, AFA0908-250, AFA0908-500, AFA0908-1

Atshaves BP, et al: (2004) Mol Cell Biochem. 259 (1-2), 115-29.; ; Nakamura T, et al: (2005) Diabestes Care. 28 (11), 2728-32.

Schlabritz-Loutsevitch N, et al. A first case of hepatocellular carcinoma in the baboon (Papio spp.) placenta. (J Med Primatol. 2019) {https://pubmed.ncbi.nlm.nih.gov/30246873/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Enzymes & Proteases

NP_001434

P07148

The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: FABP1 Recombinant protein ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: Liver tissues lysate ;

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human FABP1 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Flow cytometry analysis of FABP1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

ICC/IF analysis of FABP1 in Hep3B cells. The cell was stained with AFA0908 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .