Humaan Frataxin antilichaam

Frataxin, also known as FXN, is an iron binding protein which belongs to FRATAXIN family. It is an anti-apoptotic protein which prevents mitochondrial damage, reactive oxygen species production and act as either an iron chaperone or an iron storage protein. Frataxin is localized to the mitochondrion and regulating mitochondrial iron transport and respiration. Defects in this protein cause Friedreich ataxia.

CyaY, FA, FARR, FRDA, X25, FXN, Friedreich ataxia protein.

Human

Human

Recombinant human Frataxin (42-210aa) purified from E. coli

AT10E11

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Frataxin, CyaY, FA, FARR, FRDA, X25, FXN, Friedreich ataxia protein., ATGA0440-10 µg, ATGA0440-20 µg, ATGA0440-50 µg, ATGA0440-100 µg, ATGA0440-250 µg, ATGA0440-500 µg, ATGA0440-1 mg, ATGA0440-10, ATGA0440-20, ATGA0440-50, ATGA0440-100, ATGA0440-250, ATGA0440-500, ATGA0440-1

Campuzano V., et al. (1996) Science. 271 (5254) : 1423-7. ; ; Shan Y., et al. (2007) Hum Mol Genet. 16 (8) : 929-41.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_000135

Q16595

The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Frataxin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : 293T cell lysate ; ; Lane 2. : Frataxin transfected 293T cell lysate

ICC/IF analysis of Frataxin in A549 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Frataxin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .