Humaan FUBP1-antilichaam

FUBP1 is a transcriptional regulator and fulfills an important function in the precise control of c-myc transcription. The c-Myc protein is a transcription factor which regulates the transcription of many different target genes that play a role in proliferation, cell cycle progression, differentiation, apoptosis and cell metabolism. Consequently, FUBP1 is also involved in the regulation of proliferation and differentiation, as confirmed by different experimental approaches. Knockdown of FUBP1 or expression of a dominant-negative FUBP1 (DNA-binding domain lacking effector activity) led to proliferation arrest in U2OS and Saos-2 osteosarcoma cells due to reduced c-myc expression.

Far upstream element (FuSE) binding protein 1, FBP, FuBP

Human

Human

Recombinant human FUBP1 (279-448aa) purified from E.coli

AT14F5

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

FUBP1, ATGA0369-10 µg, ATGA0369-20 µg, ATGA0369-50 µg, ATGA0369-100 µg, ATGA0369-250 µg, ATGA0369-500 µg, ATGA0369-1 mg, ATGA0369-10, ATGA0369-20, ATGA0369-50, ATGA0369-100, ATGA0369-250, ATGA0369-500, ATGA0369-1

Duncan R., et al. (1994) Genes Dev. 8 (4) : 465-480.; ; Liu J., et al. (2006) EMBO J. 25 (10) : 2119-2130.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_003893

Q96AE4

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FUBP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: Jurkat cell lysate

ICC/IF analysis of FUBP1 in HeLa cells. The cell was stained with ATGA0369 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .