Humaan Gankyrine/PSMD10 antilichaam

26S proteasome non-ATPase regulatory subunit 10 is an enzyme that in humans is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.

Proteasome 26S subunit non ATPase 10 (PSMD10) isoform 1, ankyrin repeat protein p28, Gankyrin, 26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, PSMD10.

Human

Human

Recombinant human PSMD10 (1-226aa) purified from E. coli

AT1F4

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PSMD10, proteasome 26S subunit non ATPase 10 (PSMD10) isoform 1, ankyrin repeat protein p28, Gankyrin, 26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, PSMD10., ATGA0386-10 µg, ATGA0386-20 µg, ATGA0386-50 µg, ATGA0386-100 µg, ATGA0386-250 µg, ATGA0386-500 µg, ATGA0386-1 mg, ATGA0386-10, ATGA0386-20, ATGA0386-50, ATGA0386-100, ATGA0386-250, ATGA0386-500, ATGA0386-1

Hori T., et al. (1998) Gene. 216 (1) : 113-122.; ; Kaneko T., et al. (2009) Cell. 137: 914-925.; ; Wang J., et al. (2009) Cytogenet Genome Res. 125: 87-97.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Ubiquitination

NP_002805

O75832

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSMD10 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : A549 cell lysate ; ; Lane 2. : PC3 cell lysate ; ; Lane 3. : K562 cell lysate ; ; Lane 4. : HepG2 cell lysate ; ; Lane 5. : HeLa cell lysate ; ; Lane 6. : Jurkat cell lysate

ICC/IF analysis of PSMD10 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PSMD10 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .