Humaan GARS antilichaam

GARS, also known as glycyl-tRNA synthetase, is one of the aminoacyl-tRNA synthetase that charge tRNAswith their cognate amino acids. Defects in the gene encoding GlyRS is the cause of Charcot-Marie-Toothdisease type 2D (CMT2D), which is an autosomal dominant inherited disease characterized by severe weakness, atrophy and absence of deep tendon reflexes in the upper extremities. Defects in the GlyRS gene is also thecause of distal hereditary muscular neuropathy type V (HMN5), a disease similar to CMT2D, though the distalsensory involvement is less severe in HMN5 patients.

Glycyl-tRNA synthetase, CMT2D, DSMAV, GlyRS, HMN5, SMAD1

Human

Human

Recombinant human GARS (43-289aa) purified from E. coli

AT4E10

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

GARS, ATGA0557-10 µg, ATGA0557-20 µg, ATGA0557-50 µg, ATGA0557-100 µg, ATGA0557-250 µg, ATGA0557-500 µg, ATGA0557-1 mg, ATGA0557-10, ATGA0557-20, ATGA0557-50, ATGA0557-100, ATGA0557-250, ATGA0557-500, ATGA0557-1

Xie. W. (2007) Proc Natl Acad Sci U S A. 104: 9976-9981.; ; Antonellis. A. (2003) AM J Hum Genet 72: 1293-1299.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenetics and Nuclear Signaling

NP_002038

P41250

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GARS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: 293T cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: U87MG cell lysate; ; Lane 5.: A431 cell lysate;

ICC/IF analysis of GARS in HeLa cells. The cell was stained with ATGA0557 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .