Humaan GAS7 antilichaam

Growth arrest-specific 7, also known as GAS7, is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development by promoting maturation and morphological differentiation of cerebellar neurons. Inhibition of GAS7 production in terminally differentiating cultures of embryonic murine cerebellum impedes neurite outgrowth. Conversely, the hyper-expression of GAS7 may play an important role in the initiation and development of human osteosarcoma.

Growth arrest-specific 7 isoform b, KIAA0394, Growth arrest-specific 7, isoform b Growth arrest specific 7, GAS 7, GAS7, MGC1348, MLL/GAS7 fusion protein.

Human

Human

Recombinant human GAS7 (1-416aa) purified from E. coli

AT4H8

Unconjugated

ELISA, WB

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

GAS7, Growth arrest-specific 7 isoform b, KIAA0394, Growth arrest-specific 7, isoform b Growth arrest specific 7, GAS 7, GAS7, MGC1348, MLL/GAS7 fusion protein., ATGA0147-10 µg, ATGA0147-20 µg, ATGA0147-50 µg, ATGA0147-100 µg, ATGA0147-250 µg, ATGA0147-500 µg, ATGA0147-1 mg, ATGA0147-10, ATGA0147-20, ATGA0147-50, ATGA0147-100, ATGA0147-250, ATGA0147-500, ATGA0147-1

Chao CC, et al., (2005) J Neurosci Res. 81 (2) :153-62; ; She BR, et al., (2002) Exp Cell Res. 273 (1) :34-44

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1000. Recommended starting dilution is 1:500.

Neuroscience

NP_958836

O60861

The extracts of mouse brain (50 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GAS7 (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of GAS7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GAS7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of GAS7 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GAS7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0147-IF2.jpg}