Humaan geminine antilichaam

Geminin is a 25 kDa protein, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. In addition, geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions.

GMNN, Geminin, Geminin, DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, OTTHuMP00000039393, RP3 369A17.3

Human

Human

Recombinant human Geminin (1-209 aa) purified from E. coli

2H7

Unconjugated

ELISA, WB

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Geminin, AGM0621-10 µg, AGM0621-20 µg, AGM0621-50 µg, AGM0621-100 µg, AGM0621-250 µg, AGM0621-500 µg, AGM0621-1 mg, AGM0621-10, AGM0621-20, AGM0621-50, AGM0621-100, AGM0621-250, AGM0621-500, AGM0621-1

McGarry TJ. et al., (1998) Cell. 93 (6) :1043-53.; ; Pitulescu M. et al., (2005) Cell Mol Life Sci. 62 (13) :1425-33.; ; Luo L. et al., (2004) Nature. 427 (6976) :749-53.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_056979

O75496

The cell lysate of K562 (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Geminin (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.