Humaan Glutamine Synthetase antilichaam

Glutamine synthetase (GLUL), which is therefore able to regulate intracellular concentrations of glutamate. GLUL catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. GLUL is essential for proliferation of fetal skin fibroblasts and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in GLUL are associated with congenital glutamine deficiency.

Glutamine synthase, Glutamate-ammonia ligase, GLNS, Glutamate-ammonia ligase, GS, Palmitoyltransferase GLUL

Human

Human

Recombinant human GLUL (1-373aa) purified from E. coli

AT8D7

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

GLUL, Glutamine synthetase , GLNS, GS, PIG43, PIG59, cell proliferation-inducing protein 59, GLNA, GLNA_HUMAN, GLNS, GLULGlutamate ammonia ligase, Glutamate decarboxylaseGlutamate--ammonia ligase, glutamine synthaseGlutamine synthetase, GS, PIG 43, PIG 59, PIG43PIG59, Proliferation inducing protein 43, ATGA0474-10 µg, ATGA0474-20 µg, ATGA0474-50 µg, ATGA0474-100 µg, ATGA0474-250 µg, ATGA0474-500 µg, ATGA0474-1 mg, ATGA0474-10, ATGA0474-20, ATGA0474-50, ATGA0474-100, ATGA0474-250, ATGA0474-500, ATGA0474-1

Vermeulen T., et al, (2008) Arch Biochem Biophys. 478: 96-102. ; ; Liaw SH., et al. (1995) Protein Sci. 4 (11) : 2358-65.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Neuroscience

NP_001028216

P15104

The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GLUL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Mouse brain tissue lysate ; ; Lane 2.: Mouse liver tissue lysate

ICC/IF analysis of GLUL in HeLa cells. The cell was stained with ATGA0474 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0474-IF1b.jpg}