Humaan Glutathion Reductase/GSR antilichaam

Glutathione reductase (GR) also known as glutathione-disulfide reductase (GSR) is an enzyme that in humans is encoded by the GSR gene. Glutathione reductase catalyzes the reduction of glutathione disulfide (GSSG) to the sulfhydryl form glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell. Glutathione reductase functions as dimeric disulfide oxidoreductase and utilizes an FAD prosthetic group and NADPH to reduce one mole of GSSG to two moles of GSH.

Glutathione reductase, GLuR, GRD1

Human

Human

Recombinant human GSR (43-522aa) purified from E.coli

AT11D10

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Glutathione Reductase, GSR, Glutathione reductase, GLuR, GRD1, ATGA0323-10 µg, ATGA0323-20 µg, ATGA0323-50 µg, ATGA0323-100 µg, ATGA0323-250 µg, ATGA0323-500 µg, ATGA0323-1 mg, ATGA0323-10, ATGA0323-20, ATGA0323-50, ATGA0323-100, ATGA0323-250, ATGA0323-500, ATGA0323-1

Deponte M. (2013) Biochim Biophys Acta 1830 (5) : 3217-3266; ; Meister A. (1988) J Biol Chem 263 (33) : 17205-17208; ; Mannervik B. (1987) Biochem Soc Trans 15 (4) : 717-718

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Redox Proteins

NP_000628

P00390

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSR antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human GSR protein;

Flow cytometry analysis of GSR in Jurkat cells. The cell was stained with ATGA0323 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of GSR in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSR antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .