Humaan Glutathion S-transferase thèta 2/GSTT2 antilichaam

Glutathione S-transferase theta-2 (GSTT2) is an enzyme catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: Alpha, Mu, Pi, Theta, and Zeta. The theta class members GSTT1 and GSTT2 share 55% amino acid sequence identity and both are thought to have an important role in human carcinogenesis.

Glutathione S-transferase theta 2, GSTT2B

Human

Human

Recombinant human GSTT2 (1-244aa) purified from E. coli

AT10H4

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

GSTT2, Glutathione S-transferase theta 2, GSTT2B, ATGA0391-10 µg, ATGA0391-20 µg, ATGA0391-50 µg, ATGA0391-100 µg, ATGA0391-250 µg, ATGA0391-500 µg, ATGA0391-1 mg, ATGA0391-10, ATGA0391-20, ATGA0391-50, ATGA0391-100, ATGA0391-250, ATGA0391-500, ATGA0391-1

Hussey AJ., et al. (1992) Biochem J. 286 (3) : 929-935.; ; Whittington AT., et al. (1996) Genomics. 33 (1) : 105-111.; ; Webb G., et al. (1996) Genomics. 33 (1) : 121-123.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_000845

P0CG29

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSTT2 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate ; ; Lane 2.: NIH/3T3 cell lysate ; ; Lane 3.: A549 cell lysate ; ; Lane 4.: Raw264.7 cell lysate

Flow cytometry analysis of GSTT2 in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of GSTT2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSTT2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .