Humaan Glutathion Synthetase/GSS antilichaam

Glutathione synthetase, also known GSS, is the second enzyme in the glutathione biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine, to form glutathione. Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) ; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.

Glutathione synthetase, GSHS, GSH synthetase

Human

Human

Recombinant human GSS (1-474aa) purified from E. coli

AT12F2

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

GSS, Glutathione synthetase, GSHS, GSH synthetase, ATGA0422-10 µg, ATGA0422-20 µg, ATGA0422-50 µg, ATGA0422-100 µg, ATGA0422-250 µg, ATGA0422-500 µg, ATGA0422-1 mg, ATGA0422-10, ATGA0422-20, ATGA0422-50, ATGA0422-100, ATGA0422-250, ATGA0422-500, ATGA0422-1

Polekhina G., et al. (1999) EMBO J. 18: 3204-3213. ; ; Huanq Z A., et al. (2000) Biochim Biophys Acta. 1493: 48-55.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_000169

P48637

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: MDA-MB231 cell lysate ; ; Lane 2.: WiDr cell lysate ; ; Lane 3.: SW480 cell lysate ; ; Lane 4.: A427 cell lysate ; ; Lane 5.: MCF7 cell lysate ; ; Lane 6.: 293T cell lysate ; ; Lane 7.: HeLa cell lysate ; ; Lane 8.: HT1080 cell lysate ; ; Lane 9.: U87MG cell lysate ; ; Lane 10.: A549 cell lysate

ICC/IF analysis of GSS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of GSS in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0422-IF2.jpg}