Humaan Glycine N-methyltransferase/GNMT antilichaam

Ras-related protein Glycine N-methyltransferase (GNMT) catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy) . Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

Glycine N-methyltransferase, GNMT, Glycine N-methyltransferase, Glycine N-methyltransferase EC 2.1.1.20, Glycine N methyltransferase

Human

Human

Recombinant human GNMT (1-295aa) purified from E. coli.

AT5D1

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Glycine N-methyltransferase, GNMT, ATGA0370-10 µg, ATGA0370-20 µg, ATGA0370-50 µg, ATGA0370-100 µg, ATGA0370-250 µg, ATGA0370-500 µg, ATGA0370-1 mg, ATGA0370-10, ATGA0370-20, ATGA0370-50, ATGA0370-100, ATGA0370-250, ATGA0370-500, ATGA0370-1

Velichkova., et al. (2006) J Phys Chem B. 109: 8216-8219.; ; Huang YC., et al. (2008) J Gastroenterol Hepatol. 23: 1384.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_061833

Q14749

The recombinant protein (50ng) and tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human GNMT protein; ; Lane 2.: Mouse Liver tissue lysate The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: GNMT Transfected 293T cell lysate {ATGA0370-WB2.jpg}

ICC/IF analysis of GNMT in HepG2 cells. The cell was stained with ATGA0370 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .