Humaan GOT2 antilichaam

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. Also, GOT2 is a major participant in the malate-aspartate shuttle, which is a passage from the cytosol to the mitochondria. The two enzymes are homodimeric and show close homology. GOT2 has been seen to have a role in cell proliferation, especially in terms of tumor growth.

Aspartate aminotransferase 2 mitochondrial, Aspartate aminotransferase 2, mitochondrial, KAT4, KATIV, mitAAT

Human

Human

Recombinant human GOT2 (30-430aa) purified from E.coli

AT8B11

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

GOT2, ATGA0357-10 µg, ATGA0357-20 µg, ATGA0357-50 µg, ATGA0357-100 µg, ATGA0357-250 µg, ATGA0357-500 µg, ATGA0357-1 mg, ATGA0357-10, ATGA0357-20, ATGA0357-50, ATGA0357-100, ATGA0357-250, ATGA0357-500, ATGA0357-1

Yang H., et al. (2015) The EMBO journal. 34 (8) : 1110-1125.; ; Guidetti P., et al. (2007) Journal of neurochemistry. 102 (1) : 103-111.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_002071

P00505

The recombinant human GOT2 (50ng), cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: GOT2 Recombinant protein; ; Lane 2.: Ramos cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: Liver tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: Balb/3T3 cell lysate {ATGA0357-WB2.jpg} The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human GOT1; ; Lane 2.: Recombinant Human GOT2 {ATGA0357-WB3.jpg}

Flow cytometry analysis of GOT2 in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of GOT2 in HeLa cells. The cell was stained with ATGA0357 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .