Humaan GRP78/HSPA5 antilichaam

Binding immunoglobulin protein (BIP) also known as 78kDa glucose-regulated protein (GRP-78) or heat shock 70kDa protein 5 (HSPA5) is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains them in a state competent for subsequent folding and oligomerization. BIP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. BIP is an abundant protein under all growth conditions, but its synthesis is markedly induced under conditions that lead to the accumulation of unfolded polypeptides in the ER.

Heat shock protein family A member 5, Heat shock 70kD protein 5, HSP70 family protein 5, Glucose-regulated protein 78kD, Binding-immunoglobulin protein, BiP, Endoplasmic reticulum chaperone BiP, Glucose-regulated protein 78kDa, Immunoglobulin heavy chain-binding protein

Human

Human

Recombinant human BIP (20-650aa) purified from Baculo virus

AT3D2

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

GRP78, HSPA5, Binding immunoglobulin protein, HSPA5, heat shock 70kDa protein 5, GRP78, glucose-regulated protein 78kD, MIF2, ATGA0320-10 µg, ATGA0320-20 µg, ATGA0320-50 µg, ATGA0320-100 µg, ATGA0320-250 µg, ATGA0320-500 µg, ATGA0320-1 mg, ATGA0320-10, ATGA0320-20, ATGA0320-50, ATGA0320-100, ATGA0320-250, ATGA0320-500, ATGA0320-1

Ting. J., et al. (1988) DNA 7 (4) : 275-86.; ; Hendershot. L.M., et al. (1994) Genomics 20 (2) : 281-284.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Apoptosis

NP_005338

P11021

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP78/HSPA5 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: MCF7 cell lysate;

ICC/IF analysis of GRP78/HSPA5 in HeLa cells. The cell was stained with ATGA0320 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of GRP78/HSPA5 in Hep3B cells. The cell was stained with ATGA0320 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0320-IF2.jpg}