Humaan GRP94/HSP90B1-antilichaam

GRP94, also known as Heat shock protein 90kDa beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells.

Tumor rejection antigen GP96, Tumor rejection antigen 1, TRA1, Stress inducible tumor rejection antigen GP96, HSP90B1, Heat shock protein 90 kDa beta member 1, GRP94, GRP 9, GP96 homolog, GP96, Glucose regulated protein 94kDa, Endothelial cell (HBMEC) glycoprotein, Endoplasmin, ECGP, 94 kDa glucose-regulated protein

Human

Human

Recombinant human GRP94 (22-803aa) purified from E.coli

AT94B9

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Tumor rejection antigen GP96, Tumor rejection antigen 1, TRA1, Stress inducible tumor rejection antigen GP96, HSP90B1, Heat shock protein 90 kDa beta member 1, GRP94, GRP 9, GRP9, GP96 homolog, GP96, Glucose regulated protein 94kDa, Endothelial cell (HBMEC) glycoprotein, Endoplasmin, ECGP, 94 kDa glucose-regulated protein, 94 kDa glucose regulated protein, 94kDa glucose regulated protein, 94kDa glucose-regulated protein, ATGA0348-10 µg, ATGA0348-20 µg, ATGA0348-50 µg, ATGA0348-100 µg, ATGA0348-250 µg, ATGA0348-500 µg, ATGA0348-1 mg, ATGA0348-010, ATGA0348-020, ATGA0348-050, ATGA0348-100, ATGA0348-250, ATGA0348-500, ATGA0348-01M

Randow F., et al. (2001) Nat Cell Biol. 3 (10) : 231.; ; Li Z., et al. (2002) Front Biosci. 7: 731-751.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_003290

P14625

The Recombinant protein (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP94 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human GRP94 ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: Jurkat cell lysate ; ; Lane 4.: HepG2 cell lysate

Flow cytometry analysis of GRP94 in U87MG cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of GRP94 in U87MG cells. The cell was stained with ATGA0348 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .