Humaan GSK-3 bèta-antilichaam

Glycogen synthase kinase 3beta (GSk3beta) is a serine/threonine kinase involved in insulin, growth factor and Wnt signaling. Glycogen synthase kinase 3beta (GSk3beta) is a molecular regulator of cell fate in the central nervous system and a target of the phosphatidylinositol 3-kinase (PI3k) pathway. It is an enzyme with a diverse number of actions in intracellular signaling systems, regulating neuronal plasticity, gene expression and cell survival.

Glycogen synthase kinase 3 beta

Human

Human

Recombinant human GSK3 beta (341-420aa) purified from E. coli

1F7

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

GSK3 b, AGK0601-10 µg, AGK0601-20 µg, AGK0601-50 µg, AGK0601-100 µg, AGK0601-250 µg, AGK0601-500 µg, AGK0601-1 mg, AGK0601-10, AGK0601-20, AGK0601-50, AGK0601-100, AGK0601-250, AGK0601-500, AGK0601-1

Piyajit W et al., (2002) Proc Natl Acad Sci 99 (12) :7951-7955.; ; Rana D et al., (2003) EMBO J 22 (3) : 494-501.; ; Patrizia D S et al., (2006) Neurobiol Aging 27 (3) : 413-422.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Enzymes & Proteases

NP_002084

P49841

The cell lysate of HeLa (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSK3 beta (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow cytometry analysis of GSK-3 beta in HeLa cells. The cell was stained with AGK0601 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of GSK-3 beta in HeLa cells. The cell was stained with AGK0601 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .