Humaan HABP1/C1QBP antilichaam

Complement component 1 Q subcomponent-binding protein, mitochondrial is a protein that in humans is encoded by the C1QBP gene. The human complement subcomponent C1q associates with C1r and C1s in order to yield the first component of the serum complement system. The protein encoded by this gene is known to bind to the globular heads of C1q molecules and inhibit C1 activation. This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein.

Complement C1q binding protein, Complement component 1, Q subcomponent binding protein, GC1QBP, Glycoprotein gC1Q-R, gC1qR, Mitochondrial matrix protein p32, ASF/SF2-associated protein p32, SF2p32, C1q globular domain-binding protein, Hyaluronan-binding protein 1, Splicing factor SF2-associated protein

Human

Human

Recombinant human C1QBP (74-282aa) purified from E.coli

AT1G7

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

HABP1, C1QBP, ATGA0330-10 µg, ATGA0330-20 µg, ATGA0330-50 µg, ATGA0330-100 µg, ATGA0330-250 µg, ATGA0330-500 µg, ATGA0330-1 mg, ATGA0330-10, ATGA0330-20, ATGA0330-50, ATGA0330-100, ATGA0330-250, ATGA0330-500, ATGA0330-1

Deb TB, et al. (1996) J Biol Chem 271 (4) :2206-12. ; ; Ghebrehiwet B, et al. (1994) J Exp Med 179 (6) :1809-21.; ; Entrez Gene: C1QBP complement component 1, q subcomponent binding protein.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Hormones & Cytokines

NP_001203

Q07021

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human C1QBP antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Recombinant protein ; ; Lane 2 : HeLa cell lysate ; ; Lane 3 : Jurkat cell lysate ; ; Lane 4 : MCF7 cell lysate ; ; Lane 5 : Ramos cell lysate ; ; Lane 6 : 293T cell lysate ; ; Lane 7 : A549 cell lysate

ICC/IF analysis of C1QBP in HeLa cells. The cell was stained with ATGA0330 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .