Humaan Hexokinase 1/2 antilichaam

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.

BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase-1, Hexokinase-2, Hexokinase-A, HK I, HK II, HK1, HK1 tb, HK1-s, HK1-ta, HK1-tc, HK2, HXK1, mHk1-s, Muscle form hexokinase, Tumor isozyme

Human

Human

Recombinant human Hexokinase 2 (1-917aa) purified from E. coli

1A7

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase 1, Hexokinase 2, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase1, Hexokinase-1, Hexokinase2, Hexokinase-2, Hexokinase-A, HK I, HK II, HK1, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HK2, HKI, HKII, HXK 1, HXK 2, HXK1, HXK-1, HXK2, HXK-2, mHk1-s, Muscle form hexokinase, tumor isozyme, AHK0812-10 µg, AHK0812-20 µg, AHK0812-50 µg, AHK0812-100 µg, AHK0812-250 µg, AHK0812-500 µg, AHK0812-1 mg, AHK0812-010, AHK0812-020, AHK0812-050, AHK0812-100, AHK0812-250, AHK0812-500, AHK0812-01M

Furuta H, et al., (1996) Genomics 36 (1) :206-209.; ; Jon E, et al., (2003) J Exp Biology 206:2049-2057.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_000180

P52789

The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase 1/2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Hexokinase 1 recombinant protein; ; Lane 2.: Hexokinase 2 recombinant protein; ; Lane 3.: Hexokinase 3 recombinant protein; ; Lane 4.: Hexokinase 4 recombinant protein The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase 1/2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: K562 cell lysate; ; Lane 2.: Jurkat cell lysate{AHK0812-WB2.jpg}

Flow cytometry analysis of Hexokinase 1/2 in Jurkat cells. The cell was stained with AHK0812 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of Hexokinase 1/2 in Jurkat cells. The cell was stained with AHK0812 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .