Humaan HINT2-antilichaam

Histidine triad nucleotide binding protein 2 (HINT2) is a mitochondrial protein. This protein is an AMP-lysine hydrolase and phosphoamidase and may contribute to tumor suppression. This protein is a member of the HIT superfamily and Hint subfamily, which are characterized as nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides. HINT2 regulates steroidogenesis through calcium-dependent and calcium-independent signalling pathways that may serve to maintain a favorable mitochondrial potential. Its role in calcium homeostasis may also contribute to its proapoptotic function in hepatocytes and other non-steroidogenic cells, though the exact mechanism remains unclear.

Histidine triad nucleotide-binding protein 2, HIT-17

Human

Human

Recombinant human HINT2 (18-163aa) purified from E. coli

AT5B10

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

HINT2, Histidine triad nucleotide-binding protein 2, HIT-17, ATGA0378-10 µg, ATGA0378-20 µg, ATGA0378-50 µg, ATGA0378-100 µg, ATGA0378-250 µg, ATGA0378-500 µg, ATGA0378-1 mg, ATGA0378-10, ATGA0378-20, ATGA0378-50, ATGA0378-100, ATGA0378-250, ATGA0378-500, ATGA0378-1

Maize KM., et al. (2013) The FEBS journal. 280 (14) : 3389-3398.; ; Lenglet S., et al. (2008) Endocrinology. 149 (11) : 5461-5469.; ; Martin J., et al. (2006) Gastroenterology. 130 (7) : 2179-2188.; ; Lee LR., et al. (2013) Cancer prevention research. 6 (7) : 731-743.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_115982

Q9BX68

The recombinant human HINT2 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HINT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HINT2 Recombinant protein ; ; Lane 2. : HepG2 cell lysate ; ; Lane 3. : U87MG cell lysate