Humaan HIP/ST13 antilichaam

HIP (Hsc70-interacting protein), also known as ST13, is a co-chaperone to the major heat shock proteins, HSP70 and HSP90, and appears in early receptor complexes. Through mutual binding to both HSP70 and HSP90, Hip functions as an adaptor that can integrate HSP70 and HSP90 interactions. Also, Hip has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones.

Hsc70-interacting protein, ST13, HOP, SNC6, FAM10A1, Hsc70-interacting protein

Human

Human

Recombinant human HIP/ST13 (1-369aa) purified from E. coli

AT5C6

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

ST13, Hsc70-interacting protein, ST13, HOP, SNC6, FAM10A1, Hsc70-interacting protein, ATGA0309-10 µg, ATGA0309-20 µg, ATGA0309-50 µg, ATGA0309-100 µg, ATGA0309-250 µg, ATGA0309-500 µg, ATGA0309-1 mg, ATGA0309-10, ATGA0309-20, ATGA0309-50, ATGA0309-100, ATGA0309-250, ATGA0309-500, ATGA0309-1

Nelson GM, et al., (2004) Mol Endocrinol.18 (7) : 1620-30; ; Johnson BD, et al., (1998) J Biol Chem. 273 (6) :3679-86

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Heat Shock Proteins

NP_003923

P50502

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ST13 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: SW480 cell lysate ; ; Lane 4.: Jurkat cell lysate ; ; Lane 5.: K562 cell lysate ; ; Lane 6.: LnCap cell lysate ; ; Lane 7.: HeLa cell lysate ; ; Lane 8.: PC3 cell lysate

Flow cytometry analysis of ST13 in 293T cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . Flow cytometry analysis of ST13 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {ATGA0309-FC2.jpg}

ICC/IF analysis of ST13 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of ST13 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0309-IF2.jpg} ICC/IF analysis of ST13 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ST13 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0309-IF3.jpg}