Humaan HO-1/HMOX1/HSP32 antilichaam

Heme oxygenase 1 belongs to the heme oxygenase family and is an essential enzyme in heme catabolism. It cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Also this protein is known to play an important role in the regulation of cardiovascular function and its adaptive response to a variety of stressors.

HO-1, Heat shock protein 32, HSP32, bK286B10, D8Wsu38e, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, Hmox, HMOX 1, HMOX1, HO, HO 1, HO1.

Human

Human

Recombinant human Heme oxygenase1 (1-266aa) purified from E. coli

AT1D6

Unconjugated

ELISA, WB, FACS, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

HO1, HMOX1, HSP32, HO-1, Heat shock protein 32, HSP32, bK286B10, D8Wsu38e, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, Hmox, HMOX 1, HMOX1, HO, HO 1, HO1., ATGA0454-10 µg, ATGA0454-20 µg, ATGA0454-50 µg, ATGA0454-100 µg, ATGA0454-250 µg, ATGA0454-500 µg, ATGA0454-1 mg, ATGA0454-10, ATGA0454-20, ATGA0454-50, ATGA0454-100, ATGA0454-250, ATGA0454-500, ATGA0454-1

Soares MP., et al. (2001) Immunol Rev.; ; Vareille M., et al. (2008) J Immunol. 180 (8) : 5720-6.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Neuroscience

NP_002124

P09601

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HMOX antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: A549 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: HepG2 cell lysate ; ; Lane 5.: TF-1 cell lysate

Flow cytometry analysis of HMOX in HeLa cells. The cell was stained with ATGA0454 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of HMOX in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0454-IF1b.jpg}ICC/IF analysis of HMOX in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0454-IF2a.jpg} {ATGA0454-IF2b.jpg}