Humaan HPRT antilichaam

HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis.

Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT, Hypoxanthine-guanine phosphoribosyltransferase HPRT 1, HPRT1, Hypoxanthine guanine phosphoribosyltransferase, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome), Hypoxanthine phosphoribosyltransferase 1.

Human

Human

Recombinant human HPRT (1-218aa) purified from E. coli

AT2G8

Unconjugated

ELISA, WB

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

HPRT, ATGA0204-10 µg, ATGA0204-20 µg, ATGA0204-50 µg, ATGA0204-100 µg, ATGA0204-250 µg, ATGA0204-500 µg, ATGA0204-1 mg, ATGA0204-10, ATGA0204-20, ATGA0204-50, ATGA0204-100, ATGA0204-250, ATGA0204-500, ATGA0204-1

Sculley DG, et al. (1993) . Hum. Genet. 90 (3) : 195-207.; ; Stout JT, Caskey CT (1986) . Annu. Rev. Genet. 19: 127-48.; ; Davidson BL, et al. (1991) . Am. J. Hum. Genet. 48 (5) : 951-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_000185

P00492

The cell lysates of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT (1:500~1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.