Humaan HPRT antilichaam

HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis.

Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT, Hypoxanthine-guanine phosphoribosyltransferase HPRT 1, HPRT1, Hypoxanthine guanine phosphoribosyltransferase, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome), Hypoxanthine phosphoribosyltransferase 1.

Human

Human

Recombinant human HPRT (1-218aa) purified from E. coli.

AT1D9

Unconjugated

ELISA, WB, FACS, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

HPRT, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT, Hypoxanthine-guanine phosphoribosyltransferase HPRT 1, HPRT1, Hypoxanthine guanine phosphoribosyltransferase, Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome), Hypoxanthine phosphoribosyltransferase 1., ATGA0496-10 µg, ATGA0496-20 µg, ATGA0496-50 µg, ATGA0496-100 µg, ATGA0496-250 µg, ATGA0496-500 µg, ATGA0496-1 mg, ATGA0496-10, ATGA0496-20, ATGA0496-50, ATGA0496-100, ATGA0496-250, ATGA0496-500, ATGA0496-1

Sculley DG, et al. (1993) . Hum. Genet. 90 (3) : 195-207.; ; Stout JT, Caskey CT (1986) . Annu. Rev. Genet. 19: 127-48.; ; Davidson BL, et al. (1991) . Am. J. Hum. Genet. 48 (5) : 951-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_000185

P00492

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: MCF7 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: A549 cell lysate

Flow cytometry analysis of HPRT in A549 cells. The cell was stained with ATGA0496 at 2-5ug for 1x106cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of HPRT in HaLe cells. The cell was stained with ATGA0496 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0496-IF1b.jpg}