Humaan HSP40/DNAJB1-antilichaam

Human Hsp40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. Hsp40 protein contains the J-domain which controls interaction with the ATPase domain of Dnak.

Heat shock 40 kDa protein 1, DNAJB1, DNAJ1, HDJ1, HSPF1, HSP40 (DNAJ), Heat shock 40 kDa protein 1, Heat shock protein 40, HSP40, DnaJ protein homolog 1, HDJ-1, DnaJ homolog subfamily B member 1, Heat shock 40 kDa protein 1, radial spoke 16 homolog B (Chlamydomonas), Hsp40, Sis1, RSPH16B

Human

Human

Recombinant human Hsp40 (1-340aa) purified from E. coli

K1C7

Unconjugated

ELISA, WB

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

HSP40, DNAJB1, Heat shock 40 kDa protein 1, DNAJB1, DNAJ1, HDJ1, HSPF1, HSP40 (DNAJ), Heat shock 40 kDa protein 1, Heat shock protein 40, HSP40, DnaJ protein homolog 1, HDJ-1, DnaJ homolog subfamily B member 1, Heat shock 40 kDa protein 1, radial spoke 16 homolog B (Chlamydomonas), Hsp40, Sis1, RSPH16B, AHS0716-10 µg, AHS0716-20 µg, AHS0716-50 µg, AHS0716-100 µg, AHS0716-250 µg, AHS0716-500 µg, AHS0716-1 mg, AHS0716-10, AHS0716-20, AHS0716-50, AHS0716-100, AHS0716-250, AHS0716-500, AHS0716-1

Hata M et al., (1996) Genomics 38 (3) :446-449; ; Manishkumar et al., (2005) J Biol Chem 280 (48) :40041-40050; ; Christine M et al., (2007) Genetics 175 (4) :1649-1664

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

Heat Shock Proteins

NM_006145

P25685

The Cell lysates of Jurkat (50 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: HSP40 Transfected 293T cell lysate {AHS0716-WB2.jpg}