Humaan HSP60 antilichaam

Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kDa mammalian equivalent to GroEL of E. coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding.

Heat shock 60kDa protein 1, CPN60, GroEL, HSP65, SPG13, HuCHA60, Heat shock 60kDa protein 1, HSP60, Heat shock 60kDa protein 1 60 kDa chaperonin, GroEL, E, coli, homolog of, 60 kDa heat shock protein mitochondrial, 60kDa, cb863, Chaperonin, Chaperonin 60, Chaperonin, 60-KD, CPN 60, fa04a05, fb22d10, fi27b05, GroEL Homolog, Heat shock 60kD protein 1 (chaperonin), Heat shock 60kD protein 1 chaperonin, heat shock 60kDa protein 1 (chaperonin), Heat shock protein 1 (chaperonin), Heat Shock Protein 60, Heat shock protein 65, HLD4, Hsp 60, HSP 65, HSPD 1, HSPD1, HuCHA60, id:ibd2197, Spastic paraplegia 13 Mitochondrial matrix protein P1, P60 lymphocyte protein, sb:cb144, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (autosomal dominant), SPG 13, wu:fa04a05, wu:fb22d10, wu:fi04a12, wu:fi27b05.

Human

Human

Recombinant human Hsp60 (1-573aa) purified from E. coli

20000

Unconjugated

ELISA, WB, ICC/IF, IHC

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

HSP60, AHS0815-10 µg, AHS0815-20 µg, AHS0815-50 µg, AHS0815-100 µg, AHS0815-250 µg, AHS0815-500 µg, AHS0815-1 mg, AHS0815-10, AHS0815-20, AHS0815-50, AHS0815-100, AHS0815-250, AHS0815-500, AHS0815-1

Cheng MY, et al., (1990) Nature. 348:455-8; ; Ghosh JC, et al., (2008) J Biol Chem. 283 (8) :5188-94

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Heat Shock Proteins

NP_002147

P10809

Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp60 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: A549 cell lysate

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

ICC/IF analysis of Hsp60 in HeLa cells. The cell was stained with AHS0815 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .