Humaan HSP90 alfa antilichaam

Hsp90 is a human heat shock protein. In response to adverse change in their environment, cell from all organisms increase the expression of a class of proteins referred to as heat shock or stress protein. The Hsp90, a highly conserved stress-induced protein, is abundantly expressed in most tissues under non-stress conditions and is required for eukaryotic cell viability. Hsp90 is primarily a cytoplasmic protein and its function remains unknown. It exists in a dimeric form and has been observed to bind to several other cellular proteins such as retro-virus kinases, steroid receptor, heme-regulated protein kinase, actin and tubulin.

Heat shock protein HSP 90-alpha, HSP 86, Renal carcinoma antigen NY-REN-38, HSPC1, HSPCA, Heat shock protein 90-alpha Renal carcinoma antigen NY REN 38, D6S182, FLJ26984, FLJ31884, Heat shock 90kDa protein 1 alpha, heat shock protein 90kDa alpha (cytosolic), class A member 2, HSP84, HSP86, Hsp89, HSP89A, Hsp90, HSP90A, HSP90AA1, HSP90ALPHA, HSP90N, HSPCAL3, HSPCB, HSPN, LAP2, Lipopolysaccharide associated protein2, LPS associated protein 2, NY REN 38 antigen, hsp90, Heat shock protein HSP 90-alpha

Human

Human

Recombinant human Hsp90 (1-732aa) purified from E. coli

4F10

Unconjugated

ELISA, WB, ICC/IF, IHC, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

HSP90 a, AHS0704-10 µg, AHS0704-20 µg, AHS0704-50 µg, AHS0704-100 µg, AHS0704-250 µg, AHS0704-500 µg, AHS0704-1 mg, AHS0704-10, AHS0704-20, AHS0704-50, AHS0704-100, AHS0704-250, AHS0704-500, AHS0704-1

B-T, et al., (1984) Molecular & Cellular Biol 4: 2802-2810.; ; Pritchard, K. A, et al., (2001) J Biol Chem 276:17621-17624.; ; Miyamoto, A, et al., (2002) Nature 416:865-869.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Heat Shock Proteins

NP_005339

P07900

The cell lysates (20 µg) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate;

Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Flow cytometry analysis of HSP90 in HepG2 cells. The cell was stained with AHS0704 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of HSP90 in HeLa cells. The cell was stained with AHS00704 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .