Humaan IL-32 antilichaam

The gene encoding IL32 is located on human chromosome 16p13. 3 and is organized into eight exons. IL32 is mainly expressed in natural killer cells, T cells, epithelial cells, and blood monocytes. It can induce the proinflammatory cytokines TNFalpha and IL1beta in murine peritoneal macrophages as well as in phorbol ester-differentiated human THP1 cells. In the inflamed lesions of chronic pancreas, the ductal expression of IL32 was markedly increased. Pancreatic duct cells are the local source of IL32, and IL32 may play an important role in inflammatory responses and pancreatic cancer growth. Recently, a synergism between IL32 and other well characterized players in innate immunity has been documented.

Interleukin 32, Natural killer cells protein 4, Natural killer cell transcript 4, NK4, Tumor necrosis factor alpha-inducing factor, TAIF, TAIFb, TAIFd

Human

Human

Recombinant human IL32 (1-131aa) purified from E. coli

AT2F9

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

IL32 a, ATGA0170-10 µg, ATGA0170-20 µg, ATGA0170-50 µg, ATGA0170-100 µg, ATGA0170-250 µg, ATGA0170-500 µg, ATGA0170-1 mg, ATGA0170-10, ATGA0170-20, ATGA0170-50, ATGA0170-100, ATGA0170-250, ATGA0170-500, ATGA0170-1

Nishida A, et al. (2009) J Biol Chem, 284 (26) :17868-76.; ; Kobayashi H, et al. (2009) Cytokine, 46 (3) :351-8.; ; Mabilleau G, et al. (2009) PLoS One, 4 (1) :e4173.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology

NP_001012651

P24001

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-32 alpha antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: IL-32 alpha Transfected 293T cell lysate;

ICC/IF analysis of IL32 in Jurkat cells. The cell was stained with ATGA0170 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .