Humaan IL-33 antilichaam

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.

Interleukin-33, Interleukin-1 family member 11, IL-1F11, Nuclear factor from high endothelial venules, NF-HEV, DVS27-related protein, DVS27, C9orf26

Human

Human

Recombinant human IL-33 (112-270aa) purified from E. coli

4000000000

Unconjugated

ELISA, WB, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

IL33, ATGA0555-10 µg, ATGA0555-20 µg, ATGA0555-50 µg, ATGA0555-100 µg, ATGA0555-250 µg, ATGA0555-500 µg, ATGA0555-1 mg, ATGA0555-10, ATGA0555-20, ATGA0555-50, ATGA0555-100, ATGA0555-250, ATGA0555-500, ATGA0555-1

Brint Ek, et al., (2005) J Biol Chem. 20:277 (51) .; ; Schmitz, et al., (2005) immunity. 23:479.; ; Baekkevold ES, et al., (2003) Am.J.Pathol. 163 (1) :69-90.;

Wang Y, et al. Exposure to cigarette smoke impacts myeloid-derived regulatory cell function and exacerbates airway hyper-responsiveness. (Lab Invest. 2014) {https://pubmed.ncbi.nlm.nih.gov/25365203/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cardiovascular

NP_254274

O95760

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-33 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; The cell lysate (35ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-33 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; {ATGA0555-WB2.jpg}

Flow cytometry analysis of IL-33 in Jurkat cells. The cell was stained with ATGA0555 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .