Humaan IL-6 antilichaam

Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length that plays critical roles in host defense, immune response and hematopoiesis. interleukin-6 (IL-6) produced by T cells, macrophages, fibroblasts, endothelial and other cells. IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-Beta, is important for developing Th17 responses. The IL-6 receptor is a trimeric complex composed of an IL-6-specific a chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NFIL6 transcription.

Interleukin-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IFNB2, HGF, HSF

Human

Human

Recombinant human Interleukin 6 (30-212aa) purified from E. coli

AT1F10

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

VLAB, VLA-4 subunit bet, VLA4 subunit bet, VLA 4 subunit bet, MSK12, MSK 12, MDF2, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta1, Integrin beta 1, IL-6, IL6, IL 6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, GP IIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGA0313-10 µg, ATGA0313-20 µg, ATGA0313-50 µg, ATGA0313-100 µg, ATGA0313-250 µg, ATGA0313-500 µg, ATGA0313-1 mg, ATGA0313-010, ATGA0313-020, ATGA0313-050, ATGA0313-100, ATGA0313-250, ATGA0313-500, ATGA0313-01M

Heinrich P.C., et al. (1998) Biochem J 334: 297-314; ; Heinrich P.C., et al. (1998) Z Ernahrungswiss 37: 43-49; ; Jones S.A. (2005) J Immunol 175: 3463-3468

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Hormones & Cytokines

NP_000591

P05231

The Human IL6 recombinant protein (10ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Interleukin 6 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.