Humaan Integrine beta 1/CD29 antilichaam

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.

Integrin beta 1, Integrin beta 1, CD29, FNRB, MDF2, VLAB, GPIIA, MSK12, Fibronectin receptor subunit beta, Glycoprotein Iia, GPIIA, VLA-4 subunit bet

Human

Human

Recombinant human ITGB1 (21-461aa) purified from E. coli.

AT47E2

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

VLA-BETA, VLAB, VLA-4 subunit beta, NRK2, Nicotinamide riboside kinase 2, MSK12, MIBP, MDF2, ITGB1, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta-1 isoform 1A, Integrin beta 1, Integrin b1, Il-6, IL6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGA0485-10 µg, ATGA0485-20 µg, ATGA0485-50 µg, ATGA0485-100 µg, ATGA0485-250 µg, ATGA0485-500 µg, ATGA0485-1 mg, ATGA0485-010, ATGA0485-020, ATGA0485-050, ATGA0485-100, ATGA0485-250, ATGA0485-500, ATGA0485-01M

Pellinen T., et al. (2008) Dev. Cell. 15: 371-385.; ; Chuang N.N., et al. (2007) Biochem. Soc. Trans. 35:1292-1294.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Neuroscience

NP_002202

P05556

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ITGB1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: A549 cell lysate ; ; Lane 2.: PC3 cell lysate ; ; Lane 3.: HT1080 cell lysate

ICC/IF analysis of ITGB1 in HeLa cells. The cell was stained with ATGA0485 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0485-IF1b.jpg}