Humaan ISG15/UCRP antilichaam

ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play important roles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15 is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety of proteins when cells are treated with type I interferon or lipopolysaccharide.

Interferon-stimulated gene 15 kDa ubiquitin-like modifier, GIP2, uCRP, IFI15, ISG15, Interferon-stimulated gene, 15 kDa ubiquitin-like modifier, Interferon-stimulated gene, 15 kDa ubiquitin-like modifier IFI 15, Interferon alpha inducible protein, Interferon induced 15 kDa protein, Interferon induced 17 kDa protein, Interferon induced 17 kDa/15 kDa protein, Interferon Stimulated Gene 15, Interferon stimulated protein 15 kDa, ISG 15, ISG15 ubiquitin like modifier, ubiquitin cross reactive protein precursor

Human

Human

Recombinant human ISG15 (1-157aa) purified from E. coli

300000

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

ISG15, UCRP, AIS0701-10 µg, AIS0701-20 µg, AIS0701-50 µg, AIS0701-100 µg, AIS0701-250 µg, AIS0701-500 µg, AIS0701-1 mg, AIS0701-10, AIS0701-20, AIS0701-50, AIS0701-100, AIS0701-250, AIS0701-500, AIS0701-1

Jonathan D et al., (1996) Proc Natl Acad Sci USA 93:211-215.; ; Weiming Y et al., (2001) EMBO J 20 (3) :362-371.; ; Chen Z et al., (2005) Proc Natl Acad Sci USA 102 (29) :10200-10205.

Zahoor MA, et al. HIV-1 Vpr Induces Interferon-Stimulated Genes in Human Monocyte-Derived Macrophages. (PLoS One. 2014) {https://pubmed.ncbi.nlm.nih.gov/25170834/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Ubiquitination

NP_005092

P05161

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15/UCRP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : HeLa cell lysate; ; Lane 2. : MCF7 cell lysate

ICC/IF analysis of ISG15 in HeLa cells. The cell was stained with AIS0701 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .