Humaan ISG15/UCRP antilichaam

ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play importantroles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety ofproteins when cells are treated with type I interferon or lipopolysaccharide.

Interferon-stimulated gene 15 kDa ubiquitin-like modifier, GIP2, uCRP, IFI15, ISG15, Interferon-stimulated gene, 15 kDa ubiquitin-like modifier, Interferon-stimulated gene,15 kDa ubiquitin-like modifier IFI 15, Interferon alpha inducible protein, Interferon induced 15 kDaprotein, Interferon induced 17 kDa protein, Interferon induced 17 kDa/15 kDa protein, InterferonStimulated Gene 15, Interferon stimulated protein 15 kDa, ISG 15, ISG15 ubiquitin like modifier, ubiquitin cross reactive protein precursor

Human

Human

Recombinant human ISG15 (1-157aa) purified from E. coli

300000

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

ISG15, UCRP, ATGA0543-10 µg, ATGA0543-20 µg, ATGA0543-50 µg, ATGA0543-100 µg, ATGA0543-250 µg, ATGA0543-500 µg, ATGA0543-1 mg, ATGA0543-10, ATGA0543-20, ATGA0543-50, ATGA0543-100, ATGA0543-250, ATGA0543-500, ATGA0543-1

Jonathan D et al., (1996) Proc Natl Acad Sci USA 93:211-215.; ; Weiming Y et al., (2001) EMBO J 20 (3) :362-371.; ; Chen Z et al., (2005) Proc Natl Acad Sci USA 102 (29) :10200-10205.;

Zahoor MA, et al. HIV-1 Vpr Induces Interferon-Stimulated Genes in Human Monocyte-Derived Macrophages. (PLoS One. 2014) {https://pubmed.ncbi.nlm.nih.gov/25170834/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenetics and Nuclear Signaling

NP_005092

P05161

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ISG15 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: MCF7 cell lysate; ; Lane 2.: PC3 cell lysate;

ICC/IF analysis of ISG15 in HeLa cells. The cell was stained with ATGA0543 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .