Humaan Jak2-antilichaam

JAK2 is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.

Janus kinase 2, Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), JTK10

Human

Human

Recombinant human JAK2 (1014-1132aa) purified from E. coli

AT53B7

Unconjugated

ELISA, WB, FACS, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Jak2, Janus kinase 2, Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), JTK10, ATGA0421-10 µg, ATGA0421-20 µg, ATGA0421-50 µg, ATGA0421-100 µg, ATGA0421-250 µg, ATGA0421-500 µg, ATGA0421-1 mg, ATGA0421-10, ATGA0421-20, ATGA0421-50, ATGA0421-100, ATGA0421-250, ATGA0421-500, ATGA0421-1

Bole-Feycot C., et al. (1998) Endocr Rev. 19 (3) : 225-68. doi:10.1210/er.19.3.225.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cell Cycle

NP_004963

O60674

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human JAK2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : K-562 cell lysate ; ; Lane 2. : TF-1 cell lysate ; ; Lane 3. : 293T cell lysate ; ; Lane 4 : Jurkat cell lysate ; ; Lane 5 : THP-1 cell lysate

Flow cytometry analysis of JAK2 in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of JAK2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human JAK2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .