Humaan JAM-B/VE-JAM antilichaam

JAM2, also known as CD322, belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. JAM2 is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs.

Junctional adhesion molecule B, C21orf43, CD322, JAM-B, JAMB, PRO245, VE-JAM, VEJAM

Human

Human

Recombinant human JAM2 (21-238aa) purified from E. coli

AT21D7

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

JAM-B, VE-JAM, ATGA0506-10 µg, ATGA0506-20 µg, ATGA0506-50 µg, ATGA0506-100 µg, ATGA0506-250 µg, ATGA0506-500 µg, ATGA0506-1 mg, ATGA0506-10, ATGA0506-20, ATGA0506-50, ATGA0506-100, ATGA0506-250, ATGA0506-500, ATGA0506-1

Zhang Z., et al. (2004) Protein Sci. 13:2819-2824.; ; Liu T., et al. (2005) J. Proteome Res. 4:2070-2080.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_067042

P57087

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human JAM-B antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: K562 cell lysate ; ; Lane 2.: LNCaP cell lysate; ; Lane 3.: A549 cell lysate