Humaan KCTD15-antilichaam

KCTD15 (Potassium channel tetramerisation domain containing 15, also known as BTB/POZ domain-containing protein KCTD15) is protein that in humans is encoded by the KCTD15 gene. KCTD15 is expressed at high level in brain and hypothalamus. The potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels.

BTB/POZ domain-containing protein KCTD15, MGC25497, MGC2628, potassium channel tetramerisation domain containing 15

Human

Human

Recombinant human KCTD15 (1-234) purified from E.coli

AT4C3

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

KCTD15, ATGA0178-10 µg, ATGA0178-20 µg, ATGA0178-50 µg, ATGA0178-100 µg, ATGA0178-250 µg, ATGA0178-500 µg, ATGA0178-1 mg, ATGA0178-10, ATGA0178-20, ATGA0178-50, ATGA0178-100, ATGA0178-250, ATGA0178-500, ATGA0178-1

Willer CJ, et al. (2010) Nat. Genet, 41 (1) :24-34.; ; Elks CE, et al. (2010) PLoS Med, 7 (5) :e1000284.; ; Strausberg RL, et al. (2002) Proc. Natl. Acad. Sci. U.S.A, 99 (26) :16899-903.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_076981

Q96SI1

The cell lysate of HeLa (35ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human KCTD15 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KCTD15 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : 293T cell lysate; ; Lane 2 : KCTD15 Transfected 293T cell lysate {ATGA0178-WB2.jpg}

Immunofluorescence of human HeLa cells stained with monoclonal anti-human KCDT15 antibody (1:500) with Texas Red (Red) . Nucleus was stained by Hoechst 33342 (Blue) .