Humaan KIR2DL1/KIR2DL3/KIR2DS4 antilichaam

The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human Nk cells and a subset of human T cells which they are involved in recognition of either MHC class I molecules or unknown ligand on target cells and inhibit cytotoxic activities.

Killer cell immunoglobulin like receptor two Ig domains and long cytoplasmic tail 1, Killer cell immunoglobulin-like receptor 2DL1, CD158 antigen-like family member A, Natural killer-associated transcript 1, NKAT-1, p58 natural killer cell receptor clones CL-42/47.11, p58 NK receptor CL-42/47.11, p58.1 MHC class-I-specific NK receptor, CD158a

Human

Human

Recombinant human kIR2DL1 (23-223 aa) purified from E. coli

2F9

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

KIR2DL3, P43628, NP_056952.2, KIR2DS4, P43632, NP_036446, Killer cell immunoglobulin-like receptor 2DL3, CD158 antigen-like family member B2, KIR-023GB, Killer inhibitory receptor cl 2-3, NKAT2a, NKAT2b, Natural killer-associated transcript 2, NKAT-2, p58 natural killer cell receptor clone CL-6, p58 NK receptor CL-6, p58.2 MHC class-I-specific NK receptor, CD158b2, KIRCL23, Killer cell immunoglobulin-like receptor 2DS4, CD158 antigen-like family member I, Natural killer-associated transcript 8, NKAT-8, P58 natural killer cell receptor clones CL-39/CL-17, p58 NK receptor CL-39/CL-17, CD158I, KKA3, KIR2DL1, CD158a, AKR0620-10 µg, AKR0620-20 µg, AKR0620-50 µg, AKR0620-100 µg, AKR0620-250 µg, AKR0620-500 µg, AKR0620-1 mg, AKR0620-010, AKR0620-020, AKR0620-050, AKR0620-100, AKR0620-250, AKR0620-500, AKR0620-01M

Shin JS, et al., 1999. Hybridoma 18:521-527.; ; Qing R. Fan, et al., 2001. Nature Immunology 2:452-460. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology

NP_055033

P43626

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: Jurkat cell lysate The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL1 (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: KIR2DL1 recombinant protein; ; Lane 2.: KIR2DL3 recombinant protein; ; Lane 3.: KIR2DS4 recombinant protein; ; Lane 4.: KIR2DL4 recombinant protein {AKR0620-WB2.jpg}

Flow cytometry analysis of KIR2DL1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

ICC/IF analysis of KIR2DL1 in Hep3B cells. The cell was stained with AKR0620 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .