Humaan KRT23 antilichaam

KRT23, also known as cytokeratin 23, is a 422 amino acid intermediate filament protein. The human KRT23 gene is located on chromosome 17q21. 2. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, and maintenance of epithelial cells structure. Cytokeratins are described to be differentiation markers in several epithelial cancers.

CK-23, CK23, Cytokeratin-23, DKFZP434G032, HAIK1, Cytokeratin 23, MGC26158, K23, K1C23, HAIK 1, Histone deacetylase inducible keratin 23, type I cytoskeletal 23

Human

Human

Recombinant human KRT23 (271-422aa) purified from E. coli

AT2F6

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

KRT23, ATGA0225-10 µg, ATGA0225-20 µg, ATGA0225-50 µg, ATGA0225-100 µg, ATGA0225-250 µg, ATGA0225-500 µg, ATGA0225-1 mg, ATGA0225-10, ATGA0225-20, ATGA0225-50, ATGA0225-100, ATGA0225-250, ATGA0225-500, ATGA0225-1

Birkenkamp-Demtroder K., et al. (2007) Mol Oncol 1 (2) : 181-195.; ; Zhang J.S., et al. (2001) Genes Chromosomes Cancer 30: 123-135.; ; Schweizer J., et al. (2006) Cell Biol 174: 169-174.; ; Rogers M.A., et al. (2004) Differentiation 72: 527-540.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cytoskeleton

NP_056330

Q9C075

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT23 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate

Flow cytometry analysis of KRT23 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

ICC/IF analysis of KRT23 in Hep3B cells. The cell was stained with ATGA0225 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .