Humaan lactaatdehydrogenase A/LDHA antilichaam

Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1) . LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle.

Lactate dehydrogenase A, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, PIG19, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59

Human

Human

Recombinant human LDHA (1-332aa) purified from E.coli

AT1A4

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Lactate Dehydrogenase A, LDHA, ATGA0574-10 µg, ATGA0574-20 µg, ATGA0574-50 µg, ATGA0574-100 µg, ATGA0574-250 µg, ATGA0574-500 µg, ATGA0574-1 mg, ATGA0574-10, ATGA0574-20, ATGA0574-50, ATGA0574-100, ATGA0574-250, ATGA0574-500, ATGA0574-1

Le A, et al. (2010) Proc Natl Acad Sci U S A, 107 (5) :2037-42.; ; Xie H, et al. (2009) Mol Cancer Ther, 8 (3) :626-35.; ; Toyoda K, et al. (2010) J Bacteriol, 191 (13) :4251-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_005557

P00338

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP60 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: A431 cell lysate;

Flow cytometry analysis of Lactate Dehydrogenase A/LDHA in HeLa cells. The cell was stained with ATGA0574 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .