Humaan Layilin/LAYN antilichaam

Layilin is a widely expressed integral membrane hyaluronan receptor and a transmembrane protein with homology to C-type lectins. This protein interacts with the ERM protein family of cytoskeletal membrane linker molecule members, including talin, ezrin, moesin, and radixin. The carboxy-terminal domain of layilin is capable of binding to the amino-terminal domain of radixin. An interdomain interaction between the amino- and the carboxy-terminal domains of radixin inhibits its ability to bind to layilin. Layilin plays an important role in cell adhesion and motility. It is important for the maintenance of the cortical structure in the cell because it mediates signals from the extracellular matrix to the cytoskeleton. In spreading cells, Layilin acts as a membrane-binding site for talin in the peripheral ruffles, and it may play a role in the migration of cells to a wound site.

Layilin isoform 1, Layilin

Human

Human

Recombinant human LAYN (22-235aa) purified from E. coli

AT18E10

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Layilin, LAYN, ATGA0295-10 µg, ATGA0295-20 µg, ATGA0295-50 µg, ATGA0295-100 µg, ATGA0295-250 µg, ATGA0295-500 µg, ATGA0295-1 mg, ATGA0295-10, ATGA0295-20, ATGA0295-50, ATGA0295-100, ATGA0295-250, ATGA0295-500, ATGA0295-1

Bono P., et al. (2005) Exp Cell Res 1308 (1) : 177-87.; ; Mark L., et al. (1998) The Journal of Cell Biology 143 (2) : 429.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cytoskeleton

NP_001245319

Q6UX15

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LAYN antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: A549 cell lysate