Humaan Leptine/OB antilichaam

Human leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. The function of leptin in regulating appetite and metabolism, as well as the possibility of using leptin as a therapeutic agent, are currently under intense investigation.

LEP, OB, OBS, Leptin, Obesity factor, Obese protein, Leptin, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Obesity, Obesity homolog mouse, Obesity Murine Homolog Leptin

Human

Human

Recombinant human Leptin (22-167aa) purified from E. coli

AT7E10

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Leptin, OB, ATGA0460-10 µg, ATGA0460-20 µg, ATGA0460-50 µg, ATGA0460-100 µg, ATGA0460-250 µg, ATGA0460-500 µg, ATGA0460-1 mg, ATGA0460-10, ATGA0460-20, ATGA0460-50, ATGA0460-100, ATGA0460-250, ATGA0460-500, ATGA0460-1

Anini Y., et al. (2003) Diabetes. 52 (2) : 252-259.; ; Jeong KJ., et al. (1999) Appl Environ Microbiol. 65 (7) : 3027-32.; ; Hyogo H., et al. (2002) J. Biol. Chem. 277 (37) : 34117-34124.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_000221

P41159

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Leptin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Leptin transfected 293T cell lysate

ICC/IF analysis of Leptin in HeLa cells. The cell was stained with ATGA0460 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .