Humaan LITAF antilichaam

Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) mediates the expression of inflammatory cytokines such as TNF-alpha in Lipopolysaccharide-induced processes. LITAF binds to STAT6B, a member of the STAT6 family forming a complex on the TNF-alpha promoter that modulates TNF activity. High levels of expression of LITAF mRNA have been observed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen.

Lipopolysaccharide-induced TNF factor, PIG7, SIMPLE, FLJ38636, TP53I7

Human

Human

Recombinant human LITAF (1-161aa) purified from E. coli

AT1F9

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

LITAF, ATGA0152-10 µg, ATGA0152-20 µg, ATGA0152-50 µg, ATGA0152-100 µg, ATGA0152-250 µg, ATGA0152-500 µg, ATGA0152-1 mg, ATGA0152-10, ATGA0152-20, ATGA0152-50, ATGA0152-100, ATGA0152-250, ATGA0152-500, ATGA0152-1

Atucchi A, et al., (2006) Inflamm Bowel Dis 13 (1) :120-121.; ; Tang X, et al., (2005) Proc Natl Acad Sci USA 102 (14) :5132-5137.; ; Tang X, et al., (2003) Proc Natl Acad Sci USA 100 (7) :4096-4101.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_004853

Q99732

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LITAF antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate;

ICC/IF analysis of LITAF in LnCap cells. The cell was stained with ATGA0152 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .