Humaan LRPAP antilichaam

Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. LRPAP1 is a receptor antagonist that interacts with several members of the low density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. LRPAP1 is present on the cell surface, where it is an effective inhibitor of ligand binding to the kidney glycoprotein 330 (gp330) yet it is most abundant in the endoplasmic reticulum lumen and may function in receptor folding and/or trafficking.

Alpha-2-macroglobulin receptor-associated protein, A2MRAP, A2RAP, HBP44, MGC138272, MRAP, RAP

Human

Human

Recombinant human LRPAP1 (35-357aa) purified from E. coli

AT8G8

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

LRPAP, ATGA0321-10 µg, ATGA0321-20 µg, ATGA0321-50 µg, ATGA0321-100 µg, ATGA0321-250 µg, ATGA0321-500 µg, ATGA0321-1 mg, ATGA0321-10, ATGA0321-20, ATGA0321-50, ATGA0321-100, ATGA0321-250, ATGA0321-500, ATGA0321-1

Kounnas. M.Z., et al. (1992) J Biol Chem 267 (29) :21162-21166.; ; Bu. G., et al. (1995) EMBO J 14 (10) :2269-2280.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_002328

P30533

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LRPAP antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse kidney tissue lysate

ICC/IF analysis of LRPAP in HeLa cells. The cell was stained with ATGA0321 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .