Humaan MafK antilichaam

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs) . LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2) . NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG or MafK) and p45. Since Maf homodimers lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. The MafK of Maf proteins is primarily expressed during development in mesenchymal and hematopoietic cells and neurons.

Transcription factor MafK, NFE2u, P18

Human

Human

Recombinant human MAFK (1-156aa) purified from E. coli

AT2F7

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

MafK, ATGA0246-10 µg, ATGA0246-20 µg, ATGA0246-50 µg, ATGA0246-100 µg, ATGA0246-250 µg, ATGA0246-500 µg, ATGA0246-1 mg, ATGA0246-10, ATGA0246-20, ATGA0246-50, ATGA0246-100, ATGA0246-250, ATGA0246-500, ATGA0246-1

Oyake, T., et al. (1996) Mol Cell Biol 16 (11) : 6083-95.; ; Toki, T., et al. (1997) Oncogene 14: 1901-1910.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_002351

O60675

The cell lysate of Jurkat (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAFK antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAFK antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Recombinant Human MAFK; ; Lane 2. : Recombinant Human MAFG; ; Lane 3. : Recombinant Human MAFF {ATGA0246-WB2.jpg}